RETRACTED ARTICLE: Antigen based detection of cystic echinococcosis in buffaloes using ELISA and Dot-EIA
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چکیده
منابع مشابه
Comparison of the Utility of Recombinant B8/2 Subunit of the Antigen B, Native Antigen, and a Commercial ELISA Kit in the Diagnosis of Human Cystic Echinococcosis
Background: Cystic echinococcosis (CE) is a helminthic disease caused by the larval form of Echinococcus granulosus. In the present study, the B8/2 subunit of antigen B (AgB) of E. granulosus was expressed in E. coli host and then applied in a diagnostic ELISA set up. Methods: The DNA sequence of AgB8/2 subunit from E. granulosus was extracted from the GenBank and codon-optimized. The target s...
متن کاملComparison of Dot-ELISA and Sandwich ELISA Diagnostic Tests in Detection of Human Hydatidosis
Hydatidosis, caused by the larval stage of Echinococcus granulosus, is one of the most important zoonosis with worldwide distribution. As its diagnosis by clinical symptoms and scanning alone is difficult and confusing, we designed the present study to achieve a sensitive and simple diagnostic method for epidemiological studies. Sera (250 samples) were collected from 90 cases of hydatidosis pro...
متن کاملNegative-ELISA using native and filtrated cystic fluid antigens to rule out cystic echinococcosis.
An increasing number of cases of echinococcosis in Thailand have been imported, probably native infections and medical transfers. Serodiagnosis is one diagnostic choice for interpreting infections before a further step is done. Due to limited antigen, indirect ELISA has been used as a negative screening test for IgG-detection to rule out echinococcosis. Native hydatid cystic fluid (HCF) antigen...
متن کاملDot-Elisa for evaluation of hydatid cyst wall, protoscoleces and hydatid cyst fluid antigens in the serodiagnosis of cystic echinococcosis.
The aim of the present study is to evaluate cyst wall and protoscolex as an alternate source of antigen in serodiagnosis of cystic echinococcosis (CE). A total of 90 blood samples, 30 each of confirmed CE cases, disease controls and healthy controls were collected. Dot-ELISA using cyst wall, protoscolex and cyst fluid were used to demonstrate anti-hydatid antibodies. The sensitivity of Dot-ELIS...
متن کاملrapid detection of toxoplasma gondii antigen in experimentally infected mice by dot- elisa
background : toxoplasmosis is a worldwide endemic disease. in congenitally infected infants and aids patients, toxoplasmosis causes high rates of morbidity and mortality. in these cases antibody detection is difficult; so detection of parasite or its components could be useful tool for early detection and following treatment of the infection. methods : sixty-three balb/c mice were injected intr...
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ژورنال
عنوان ژورنال: Journal of Parasitic Diseases
سال: 2016
ISSN: 0971-7196,0975-0703
DOI: 10.1007/s12639-016-0762-9